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KMID : 0854720100300040307
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Korean Journal of Asthma, Allergy and Clinical Immunology
2010 Volume.30 No. 4 p.307 ~ p.313
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Effects of Bacillus Calmette-Guerin (BCG) Vaccination according to Time Point of Administration on Asthma in a Murine Model
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Kim Young-Joon
Kim Ha-Jung
Kang Mi-Jin
Kwon Ji-Won
Seo Ju-Hee
Kim Byoung-Ju
Yu Jin-Ho
Hong Soo-Jong
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Abstract
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Background: Asthma is a chronic allergic disease characterized by the infiltration of eosinophils into the airway and lung. Bacille Calmette-Guerin (BCG) has been reported to be beneficial to inhibit allergic inflammation in an animal asthma model.
Objective: The aims of the study were to investigate the efficacy of BCG according to the time point of administration on asthma in a murine model.
Method: BALB/c mice were treated with BCG (1×106 CFUs) before sensitization (?5 days) or after sensitization (＋12 days) in asthma-induced BALB/c mice by ovalbumin (OVA) sensitization. Bronchial hyperresponsiveness (BHR), influx of inflammatory cells in bronchoalveolar lavage (BAL) fluid, histopathological changes in the lung and systemic immune responses were assessed.
Result: The administration of BCG before and after sensitization suppressed asthmatic responses, including BHR, OVA-specific immunoglobulin E (IgE) production, percentage of eosinophils in BALF and pulmonary eosinophilic inflammation in the murine model compared to the control group. However, total IgE, total cell count and OVA-IgG1 were significantly suppressed by BCG before sensitization only.
Conclusion: BCG vaccinations, regardless of administration time point, suppressed asthmatic responses in mice. The mechanism of effect of BCG before sensitization is thought to be balance Th1/Th2 immune responses, but, that of BCG after sensitization may be different from BCG before sensitization.
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KEYWORD
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Airway inflammation, Asthma, BCG, Mouse
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